size marker (New England Biolabs)
Structured Review
Size Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbr322+dna/pBR322+DNA-Msp+I+Digest/pm41997936-489-18-15
Average 95 stars, based on 280 article reviews
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Cleavage Assay:Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs. Article Snippet: .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs Article Snippet: Percent cleavage in enzyme alone controls was subtracted to yield final results plotted using Sigmaplot 14.5 (Systat Software, Inc., San Jose, CA, USA). . Human topoisomerase II DNA cleavage assay The formation of nicked (Nick; SSBs), linear (Lin; DSBs), and relaxed (RLX) DNA from negatively supercoiled pBR322 (substrate) was detected by separating reaction products using ethidium bromide gel electrophoresis as previously described ( 27 , 32 ). .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of Isolation:Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs. Article Snippet: .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs Article Snippet: Percent cleavage in enzyme alone controls was subtracted to yield final results plotted using Sigmaplot 14.5 (Systat Software, Inc., San Jose, CA, USA). . Human topoisomerase II DNA cleavage assay The formation of nicked (Nick; SSBs), linear (Lin; DSBs), and relaxed (RLX) DNA from negatively supercoiled pBR322 (substrate) was detected by separating reaction products using ethidium bromide gel electrophoresis as previously described ( 27 , 32 ). .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of Solvent:Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs. Article Snippet: .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs Article Snippet: Percent cleavage in enzyme alone controls was subtracted to yield final results plotted using Sigmaplot 14.5 (Systat Software, Inc., San Jose, CA, USA). . Human topoisomerase II DNA cleavage assay The formation of nicked (Nick; SSBs), linear (Lin; DSBs), and relaxed (RLX) DNA from negatively supercoiled pBR322 (substrate) was detected by separating reaction products using ethidium bromide gel electrophoresis as previously described ( 27 , 32 ). .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of Marker:Article Title: The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM Article Snippet: The 32 P labelled FP-D (B) and FP-B (C) sense strands of parent and mutated DNA fragments were incubated with CpxR~P at the following final concentrations: 0 nM, lane a and b (indicated by “–”); 100 nM, lane c; 200 nM, lane d; 400 nM, lane e; 600 nM, lane f, 800 nM, lane g. Reactions were resolved by denaturing PAGE and analysed with a Molecular Dynamics PhosphorImager. .. Labelled |
