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size marker  (New England Biolabs)


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    Structured Review

    New England Biolabs size marker
    Size Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbr322+dna/pBR322+DNA-Msp+I+Digest/pm41997936-489-18-15
    Average 95 stars, based on 280 article reviews
    size marker - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cleavage Assay:

    Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs.
    Article Snippet: .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of pBR322 DNA (NEB, Ipswich, MA, USA), and 1 mM ATP in an assay buffer [10 mM (pH 7.5), 50 mM KCL, 50 mM NaCl, 0.1 mM EDTA, 5 mM MgCl2, 2.5% glycerol] along with DMSO solvent and 0.1–100 μM compound 1a or 2a in DMSO. ..

    Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs
    Article Snippet: Percent cleavage in enzyme alone controls was subtracted to yield final results plotted using Sigmaplot 14.5 (Systat Software, Inc., San Jose, CA, USA). . Human topoisomerase II DNA cleavage assay The formation of nicked (Nick; SSBs), linear (Lin; DSBs), and relaxed (RLX) DNA from negatively supercoiled pBR322 (substrate) was detected by separating reaction products using ethidium bromide gel electrophoresis as previously described ( 27 , 32 ). .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of pBR322 DNA (NEB, Ipswich, MA, USA), and 1 mM ATP in an assay buffer [10 mM (pH 7.5), 50 mM KCL, 50 mM NaCl, 0.1 mM EDTA, 5 mM MgCl 2 , 2.5% glycerol] along with DMSO solvent and 0.1–100 μM compound 1a or 2a in DMSO. ..

    Isolation:

    Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs.
    Article Snippet: .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of pBR322 DNA (NEB, Ipswich, MA, USA), and 1 mM ATP in an assay buffer [10 mM (pH 7.5), 50 mM KCL, 50 mM NaCl, 0.1 mM EDTA, 5 mM MgCl2, 2.5% glycerol] along with DMSO solvent and 0.1–100 μM compound 1a or 2a in DMSO. ..

    Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs
    Article Snippet: Percent cleavage in enzyme alone controls was subtracted to yield final results plotted using Sigmaplot 14.5 (Systat Software, Inc., San Jose, CA, USA). . Human topoisomerase II DNA cleavage assay The formation of nicked (Nick; SSBs), linear (Lin; DSBs), and relaxed (RLX) DNA from negatively supercoiled pBR322 (substrate) was detected by separating reaction products using ethidium bromide gel electrophoresis as previously described ( 27 , 32 ). .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of pBR322 DNA (NEB, Ipswich, MA, USA), and 1 mM ATP in an assay buffer [10 mM (pH 7.5), 50 mM KCL, 50 mM NaCl, 0.1 mM EDTA, 5 mM MgCl 2 , 2.5% glycerol] along with DMSO solvent and 0.1–100 μM compound 1a or 2a in DMSO. ..

    Solvent:

    Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs.
    Article Snippet: .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of pBR322 DNA (NEB, Ipswich, MA, USA), and 1 mM ATP in an assay buffer [10 mM (pH 7.5), 50 mM KCL, 50 mM NaCl, 0.1 mM EDTA, 5 mM MgCl2, 2.5% glycerol] along with DMSO solvent and 0.1–100 μM compound 1a or 2a in DMSO. ..

    Article Title: Novel bacterial topoisomerase inhibitors: unique targeting activities of amide enzyme-binding motifs for tricyclic analogs
    Article Snippet: Percent cleavage in enzyme alone controls was subtracted to yield final results plotted using Sigmaplot 14.5 (Systat Software, Inc., San Jose, CA, USA). . Human topoisomerase II DNA cleavage assay The formation of nicked (Nick; SSBs), linear (Lin; DSBs), and relaxed (RLX) DNA from negatively supercoiled pBR322 (substrate) was detected by separating reaction products using ethidium bromide gel electrophoresis as previously described ( 27 , 32 ). .. The cleavage assay reaction mixture (20 μL total) contained 300 ng of isolated TOP2α protein, 160 ng of pBR322 DNA (NEB, Ipswich, MA, USA), and 1 mM ATP in an assay buffer [10 mM (pH 7.5), 50 mM KCL, 50 mM NaCl, 0.1 mM EDTA, 5 mM MgCl 2 , 2.5% glycerol] along with DMSO solvent and 0.1–100 μM compound 1a or 2a in DMSO. ..

    Marker:

    Article Title: The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM
    Article Snippet: The 32 P labelled FP-D (B) and FP-B (C) sense strands of parent and mutated DNA fragments were incubated with CpxR~P at the following final concentrations: 0 nM, lane a and b (indicated by “–”); 100 nM, lane c; 200 nM, lane d; 400 nM, lane e; 600 nM, lane f, 800 nM, lane g. Reactions were resolved by denaturing PAGE and analysed with a Molecular Dynamics PhosphorImager. .. Labelled pBR322 DNA digested with MspI (New England Biolabs) was used as a size marker (lane a). ..



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    Protection of DNA breakage with CAPE. Plasmid DNA with CAPE in 1 % agarose gel staining with 1 mg/ml EtBr; Lane 1 pBR322 1 µg + 1 µM CAPE + PBS 10 µl +100 µM CuCl 2 Lane 2 pBR322 1 µg + 2 µM CAPE + PBS 10 µl +100 µM CuCl 2 + 1 mM H 2 O 2 Lane 3 pBR322 1 µg + 4 µM CAPE + PBS 10 µl +100 µM CuCl 2 + 1 mM H 2 O 2 Lane 4 pBR322 1 µg + 1 mM QT + PBS 10 µl +100 µM CuCl 2 + 1 mM H 2 O 2

    Journal: bioRxiv

    Article Title: Chemical composition and antioxidant properties of Thai propolis: Role of caffeic acid phenethyl ester (CAPE)

    doi: 10.64898/2026.01.10.698791

    Figure Lengend Snippet: Protection of DNA breakage with CAPE. Plasmid DNA with CAPE in 1 % agarose gel staining with 1 mg/ml EtBr; Lane 1 pBR322 1 µg + 1 µM CAPE + PBS 10 µl +100 µM CuCl 2 Lane 2 pBR322 1 µg + 2 µM CAPE + PBS 10 µl +100 µM CuCl 2 + 1 mM H 2 O 2 Lane 3 pBR322 1 µg + 4 µM CAPE + PBS 10 µl +100 µM CuCl 2 + 1 mM H 2 O 2 Lane 4 pBR322 1 µg + 1 mM QT + PBS 10 µl +100 µM CuCl 2 + 1 mM H 2 O 2

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    Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Staining